Description
A standard pH 7.00 buffer solution is an aqueous solution designed to have a specific pH of 7.00, which is considered neutral on the pH scale. Buffers are mixtures of weak acids and their conjugated salts or weak bases and their conjugated salts that are used to keep the pH of a solution relatively constant, even when small amounts of acid or base are added.
Preparation of a pH 7.00 buffer solution:
The pH 7.00 buffer solution can be prepared using different combinations of weak acids and bases and their respective salts. One of the most common buffer systems to achieve a pH of 7.00 is the mixture of phosphoric acid (H3PO4) and dibasic potassium phosphate (K2HPO4). The equilibrium reaction between the acid and its conjugated salt can be represented as:
Dibasic potassium phosphate acts as the conjugated salt, providing phosphate ions (H2PO4-) to neutralize any excess hydrogen ions (H+) if added to the solution. Similarly, if a base is added, the phosphoric acid present in the buffer solution will neutralize the excess hydroxide ions (OH-).
Using the pH 7.00 buffer solution:
pH 7.00 buffer solution is widely used in laboratories and scientific applications due to its neutral nature. Some of the most common applications include:
pH meter calibration: pH 7.00 buffer solution is used as one of the calibration points to verify and adjust the accuracy of pH meters.
Biological and biochemical experiments: Many procedures and assays in biology and biochemistry require a neutral pH, and this buffer solution provides the right environment to conduct these investigations.
Cell and microorganism culture: In the field of biotechnology and microbiology, this buffer solution is used to prepare culture media that maintain a neutral pH.
pH Control in Chemical Reactions: The pH 7.00 buffer solution can be used to keep pH constant during pH-sensitive chemical reactions.
It is essential to follow proper procedures and use appropriate laboratory materials to prepare and use pH 7.00 buffer solutions accurately. Also, be sure to follow recommended safety practices when working with chemicals in the lab.
PRESENTATION: 250ml